Mister Mosquito presents gamers with a quirky and unconventional concept where they find themselves in the role of a six-legged mosquito dealing with the challenges of dealing with blood-sucking humans in a suburban home during the summer. This unique game combines simulation and stealth elements, offering an intriguing twist on typical gameplay. At the Yamada house, gambling addicts must hunt down three different family members: Kenichi, Kaneyo and Ren. Each of them has their own blood type, character traits and daily routine, which adds additional complexity to the gameplay.

Success depends on the player's ability to carefully observe and adapt to the varied behavior of these unexpected hosts. The main gameplay of Mister Mosquito revolves around the subtle art of bloodsucking, requiring the gamer to strategically approach the target and avoid detection. Timing is very important, as finding the right moment to land on exposed parts of the body is key to obtaining blood without arousing suspicion.

To complicate matters, users must synchronize the mosquito's feeding rate with the victim's heartbeat, which adds extra tension and difficulty to the game. Detection of a mosquito leads to a transition to combat mode, where gamers must deftly cope with a mini-game in order to calm the alarmed person. By striking relaxation points, users try to calm their unwitting victim and avoid a hostile reaction. This dual gameplay dynamic, alternating between stealth bloodsucking and clever calming maneuvers, makes for a fun and unpredictable experience.